RNA

Part:BBa_K4506043:Design

Designed by: Juan Manuel Dominguez Larrieta   Group: iGEM22_Tec-Monterrey   (2022-10-12)


sRNA for gyrA silencing


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 104
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The complete sRNA sequence (hybridization region + Hfq binding domain) was optimized for having better structural and folding characteristics according to the Score Final detailed by our team.



Source

The sRNA-mRNA pairing sequence (24 bp) was designed by our team. The HFQ binding domain was obtained from the iGEM parts registry (BBa_K1137009).


References